GPC chromatography (gel permeation chromatography) is a widely
used high performance liquid chromatography technique for molecular
weight determination of biopolymers. GPC chromatography is also
known as size exclusion chromatography (SEC).
In GPC chromatography biopolymers are separated according to
their molecular size relative to the pore size in the packing
material of the GPC chromatography column. The solvent used in GPC
chromatography may be either aqueous or organic depending on the
properties of the specific polymers.
GPC standards for calibration
Prior to GPC chromatography of a biopolymer, the columns are
calibrated with a set of well-characterized GPC standards.
The calibration curve is obtained by proper curve fitting
against the experimental observations for the GPC standards of
known molecular weight distribution. The calibration curve provides
the tool to analyze the molecular weight distribution of the
Based on the GPC standards calibration data, it is possible to
determine the peak average molecular weight, Mp, number
average molecular weight, Mn, weight average molecular
weight, Mw, and the polydispersity, D, of a given
biopolymer sample within a given level of accuracy.
Pharmacosmos supplies dextran GPC standards for calibration of
aqueous CPG chromatography columns. Please visit Pharmacosmos'
dextran website for additional information about our